This is the transcribed will of William Buckner of Cumnor Parish, Berkshire, England, 1558 (Archdeacon Berks. wills reg. D.188, LDS film #1,041,804)
The great mystery with this will is who's not in it. Several independent records indicate that Thomas Smyth, a mayor of Oxford, married Rose Buckner in 1553, daughter of William Buckner of Botley (in Cumnor parish) and Dorothy Huckvale. At first glance, the William Buckner with his wife Dorothy in this will is a pretty good candidate to be William Buckner of Botley, who is known from other records as well. Rose had died by 1586 when the widow Dorothy Buckner wrote her will, so she would naturally be missing in it, but it is odd that neither she nor her prominent husband are mentioned in this will, though John Smyth and his wife are on the Christmas list. A son William Buckner is also omitted from the present will though (he is known from his mother's will 18 years later), so it is possible that several children were omitted for some reason (his apparent cousin Thomas Buckner of Whitley did the same thing in his 1587 will). Pleasants (the farm he lived at) was also in Botley, so it's the right place and the right names. I'm still a little hesitant because of Rose's absence, but for now I'm working under the assumption that this is the same William Buckner of Botley. One reservation I have is that Richard Buckner (d. 1548) had a son William who may have been the same as this William in the will, but as this William had to have been born prior to 1520 at the latest (to have a grown, married daughter and possibly two), I think it is unlikely that this William could have been Richard Buckner's son (though still not ready to rule it out).
The other is the existence of Hugh Buckner (a bailiff), Thomas Buckner (a mercer), and George Buckner (a musician) in Oxford in the late 1580s and 1590s, as well as a John Buckner who was somehow related to George. Their birth years were probably in the 1550s or 1560s. There are numerous connections suggesting a relationship between them and Rose (Buckner) Smith, and the Cumnor parish christenings of several children of John Buckner of Botley in the 1560s make me think that John of Botley is the son of the testator and the father of those Buckners in Oxford, who would then be Rose's nephews. Given that Richard had a son named Hugh (b. 1589) who was obviously not Hugh the bailiff, Richard wasn't their father. I'm not entirely sure if Richard was the eldest (from his clear preference in Dorothy's will), but John is obviously mentioned first in his father's will. The impression from Dorothy's will and Cumnor parish records is that the sons generally did not start families until the 1570s (Richard's first was christened in 1576). However, this impression is at odds with the idea that Rose and Elizabeth were already married in the 1550s. It is possible that Richard was the late bloomer among the sons, and that one or more of his brothers started a family not long after their father's death.
There are several ambiguous personal and place names. The most significant is probably the location of the house that Thomas Buckner inherited. Others have read this as "parish of Eaton" , which is contextually satisfying, but the form of the initial letter is very difficult to reconcile with a captial 'E'. It most resembles a 'P' to me, but there isn't a "Paton" parish that I can find (Potton, Bedf., Porton, Wilts.?). The conspicuous overbar above the 'a' probably indicates abbreviation, most likely 'n' or 'm', which might suggest Panton or Ponton, both in Lincolnshire. Since this will was probated in the archdeaconry of Berks, it has to have been a parish within the archdeaconry however. Otherwise, the will would have to have been probated in a higher court. In a stretch, I could read "Fa[ring]ton", for Faringdon, Berks. - the overbar does suggest a longer abbreviation than a simple 'n' or 'm'.
The biggest corrections I would make to previous readings of this will are the names of the daughters "Mare" (otherwise read as "Maye") and "Daye" , which appear to me to be a variant of Mary and the surname Day respectively. Ronald Day was a neighbor, and it seems logical that he was a son in law of William Buckner. The list of names that are given a bushel of barley near the end are probably mostly surnames, presumably neighbors.
A couple random things to note are the archaic plural "childer" for children and the obscure legacy "to Pyp to Mother Glover Young Glover by bond". The date on the 2nd line is very obscure, but I believe I can parse it. The first letter is just "a". The next character resembles a 'y' but is actually the thorn (þ) used to represent "th", with a flourish, so it's an abbreviation of "a thousand." The next is vC, a way of writing 500 in Roman numerals (5 × 100), and then "l[..]" which is a straightforward Roman numeral for 50-something, with the last part lost in the edge of the page. It's probably lvij (57) or lviij (58), more likely the former from the length of it.
I've tried to preserve the line arrangement so anyone who wants can compare this to the original image. I don't claim perfection, and there are some things that can be interpreted different ways. The notation {ER} and such indicates a superscript abbreviation. These are common in period documents, though sometimes they can be a little idiosyncratic.
Date of will: 6 Aug [1558?]
Probate: 17 Aug 1558
People named in the will:
In the name of god amen the vj day of august in the yer of ou' lad god aþ' v C[li?] I wyllm bucknersycke in bodyof cu~nor in the counte of barks sycke in body hole of mynd do make mys[???]my last wyll & testamen[t] aft' the forme & fassyon fyrst I be queth my sole to all myghte god & to hys mother saynt mary & to all the co~peny of h[ev?]yn my body to be berryd in saynt Thomas Ile w{t}thyn the p{ar}yssh chyrch of cu~nor It~ I geve to the mother chyrch of sayd ijd It~ I geve to the hye aut' [altar] viij d It~ I geve to the chyrch of cu~nor xij d It~ I geve to John my sune v li in mony or mony worth It~ I geve to Rycherd my sune v li in mony or mony worth It~ I geve to Thomas my sune my housse that I bought in the p{ar}yssh of Paton he to ent` in medyattly aft' my desese & to have the possessyon thereof & yff hyt plese allmyghte god to take the sayd Thomas [buckner?] to hys m'cy that then the sayd house & land to remayne to Rychard my sune It~ I geve also to Thomas a kowe & oxer[?] bolo[k?] [i.e. an ox bullock] in medyattly It~ I geve to my dout' daye the ij oxson that thaye have allredy --- next page --- (this next page is laid ouut strangely and has a crease down the middle) It~ I geve to my dowt' mare v li in mony I geve ^ her allso ^ ij peut' platte's ij potyngers & ij s[aw?]s[ars?] It~ I geve to alys my dowt' v li ij ^pewt' platte's ij potyngeres & ij sawsares It~ I geve to my dowt` ^ Jone[?] v li & the lytl[?] pewt' vessell It~ I geve to ev'ry wone of John ^ buckner my [af(erased)] sunys chyld' ech of them [?] a yewe [i.e. a ewe] It~ I geve to my dowt' dayes chyld' ev'ry on of them ayewe It~ I geve to pyp my s{er}vant & to stoton my servant ech of them alame It~ geve to ech of my godchyld' a busshell barly It~ I geve to pyp to mother glou' young glou' bye bond wyllm m'ten Joh~ clarke stanyn franlyn mallor habergen dew[d?]e[r?] camlaye & father gacy eche of them a busshell barly It I geve to Joh~ Smyth & wyff & Joh~ m'ten& wyffe eche of them halffe abusshellof malt a gay[n?]st c[r]ystmas[?] next comyng all the rest of my goods & cattell I geve dorythe my wyff whome I do make my hole [?] execatryxe of thys my last wyll & [test]ament I woud that John my sune John [?p]etygrew gent Thomas buckner & Raffe[?] [?] gunnell to be my ove'see[rs] [of?] [....] my last wyll thes be[ying?] [dytend?] --- written sideways in page 2 left margin ---- It~ I wyll that yf hyt happen eny of my chyld' do dep{ar}t owt of thys my serable lyffe then ther stock to remay~e to the rest of my chyl[d'] [Item - I will that if it happen that any of my children depart out of this miserable life then their legacy is to be divided among the rest of my children.] --- written on top of 2nd page in modern hanndwriting --- [?]P 17 Aug 1558 (Reg) W Inv Reg d 188 Arch B Regs 1553-65 (A lot of inventory follows on the next two pages.)